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Original Article
04 (
02
); 061-068
doi:
10.1055/s-0038-1676909

Histopathological Changes in Breast Cancers Following Neoadjuvant Chemotherapy: Implications for Assessment of Therapy-Induced Cytological and Stromal Changes for Better Clinical Outcome and Effective Patient care

Department of Pathology, Kasturba Medical College, Manipal Academy of Higher Education, Mangalore, Karnataka, India
Department of Oral and Maxillofacial Surgery, Bharati Vidyapeeth (Deemed to be University) Dental College and Hospital, Sangli, Maharashtra, India
Department of Oral Radiology, College of Dentistry, University of Ha'il, Ha'il, Kingdom of Saudi Arabia
Department of Oral and Maxillofacial Surgery, Malla Reddy Dental College for Women, Hyderabad, Telangana, India
Department of Pediatrics and Neonatology, McMaster University, Hamilton, Ontario, Canada
Department of Oral Medicine and Radiology, Saraswati-Dhanwantari Dental College and Hospital and Postgraduate Research Institute, Parbhani, Maharashtra, India

Address for correspondence Shazima Sheereen, Department of Pathology, Kasturba Medical College, Manipal Academy of Higher Education, Mangalore, Karnataka, India. dr.shazimasheereen@gmail.com

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This article was originally published by Thieme Medical and Scientific Publishers Private Ltd. and was migrated to Scientific Scholar after the change of Publisher.

Abstract

The histopathological interpretation of a tumor still remains the gold standard for diagnosis and deciding the type of therapy. Furthermore, in the context of chemo-radio-therapies often leading to prominent cytological and stromal changes in the tumor, histopathological interpretation during treatment, thus, becomes all the more important in correctly diagnosing and grading the tumor for an effective and planned regimen of the therapy increasing prognosis and chances of survival of the affected patients. The aim of the present study was to evaluate such cytological and stromal changes rendered by the therapy during treatment in breast cancer cases. The present study was conducted over a period of 4 years from January 2014 to June 2017 at Kasturba Medical College, Mangalore, Karnataka, India wherein the clinical and histopathological details were collected for a total of 39 breast carcinoma cases during and post-therapy. Statistical analysis was done using IBM SPSS statistics 17 (Chicago, USA). Various morphological features were analyzed for their frequency and were compared with the final diagnosis using Chi-square value (χ2), paired t-test and Fischer's test. p-value < 0.05 was considered to be statistically significant. The results of the present study revealed that 32 of the 39 breast carcinoma cases changed their grades during the course of therapy (p-value < 0.05). The fundamental manifestation of the effect of therapy was an obvious decrease in tumor cellularity. Intracellular changes commonly noted after chemotherapy included nuclear enlargement, hyperchromasia and an increased N:C ratio which were found in upto 85% of the cases followed by presence of prominent nucleoli and karyorrhexis/karyolysis. The predominant stromal changes seen post-therapy included necrosis (74.4%), fibrosis (64.1%), desmoplasia (59%) and degenerative changes (33.3%). Breast cancer therapy causes morphological alterations in the cancerous as well as the surrounding healthy tissue. The histopathological interpretation in such cases, thus, requires a thorough knowledge of the cytological and stromal changes rendered by the therapy.

Keywords

therapy-induced histopathological changes
breast cancers
neoadjuvant chemotherapy
breast cancer diagnosis and treatment

Introduction

Breast cancer is the most common cancer diagnosed in women and is the second leading cause of death in women globally.1 Even though most cases occur over the age of 50 years, it is not uncommon in younger women.1,2 Breast cancer is not a single disease and comprises several entities ranging from ductal carcinoma in situ (DCIS) to a widespread metastatic disease.3 Clinical presentation and investigations such as mammography and fine-needle aspiration cytology (FNAC) often help in the diagnosis of breast cancers. However, histopathologic examination remains the better predictor for an accurate diagnosis of breast cancers. In addition, immunohistochemical markers aid in classification and are often used to guide the therapy of the disease process. Therapy and prognosis depend on the various clinical and histopathologic factors, including tumor size, type of tumor, hormonal receptor status, the type of therapy provided, and, most significantly, at what stage the cancer is diagnosed. Because of extensive advances made in the field of oncology, various modes of therapy of breast cancers are available at present including surgery, radiotherapy, chemotherapy, and, more specifically, targeted hormonal and tumor receptors targeted therapy. Surgery that may include mastectomy or, breast-conserving surgery (lumpectomy), still remains the prime modality of therapy for most of the operable breast cancers. Hormonal therapy can be a significant addition along with surgery or, chemotherapy, especially, in patients who are estrogen receptor (ER) and/or, progesterone receptor (PR) positive. Hormonal receptor status can, thus, significantly alter the modality of therapy provided and may have better outcomes. In advanced disease, prior to surgery, radiation therapy is used to reduce the tumor size. In early breast cancers, to facilitate breast conservation, chemotherapy is more commonly used both as a neoadjuvant chemotherapy and/or postoperative chemotherapy.4 In the modern era, increased number of neoadjuvant therapy-induced surgical resection specimens of tumors are being received in histopathologic evaluation laboratories. The pathologist evaluates the residual tumor both in the breast and lymph nodes. Assessment of the therapy-induced morphologic changes seen at the cytologic and stromal levels, thus, requires a thorough knowledge of the possible cytologic and stromal changes rendered by the therapy. The aim of this study was to evaluate such cytologic and stromal changes rendered by the therapy in breast cancer cases. This ensures the role of pathologists in the correct diagnosis as well as grading of the tumor with a correct histopathologic interpretation for an effective and planned regimen of the therapy increasing prognosis and chances of survival of the affected patients rendering better clinical outcome and an effective patient care.

Materials and Methods

This study was a combined retrospective and prospective study conducted over a period of 4 years from January 2014 to June 2017 at Kasturba Medical College, Mangalore, Karnataka, India. Clinical and histopathologic details were collected from a total of 39 cases of breast carcinoma before and post-therapy, and the changes induced by the therapy were correlated. All the patients who had been diagnosed with stages II and III (T1–T4, N0–2, M0) carcinoma breast were included in the study while patients already treated with chemo- or hormonal therapy and patients who only underwent surgery without chemo- or hormonal therapy and wherein biopsy samples were found to be inadequate were excluded. The required clinical data of the patients, their lymph node status, and investigation reports (mammography, FNAC, biopsy, ER/PR status, Her2/neu status) were collected before the start of the planned therapy. The initial biopsies were subjected to routine formalin fixation and paraffin processing with microscopic analysis on hematoxylin and eosin–stained sections supplemented by special stains including immunohistochemistry to decide the histologic types and grades of the tumor and hormonal status. Subsequently, when the patients received two to six cycles of chemotherapy or, hormonal therapy, histopathologic (gross and microscopic) and immunohistochemical examination was performed and all the details pertaining to microscopic and clinical findings along with immunohistochemical findings were recorded and the changes induced by therapy were correlated.

Statistical Analysis

Statistical analysis was done using IBM SPSS statistics 17 (Chicago, Illinois, USA). The data were analyzed by proportions, tables, and graphs whereas various morphologic features were analyzed for their frequency and were compared with the final diagnosis using chi-square value (χ2), paired t-test, and Fischer's test. p < 0.05 was considered to be statistically significant whereas p < 0.001 was considered as highly significant.

Results

This study consisted of 39 breast carcinoma cases. The median age of patients was found to be 55 years. The most common chief complaint was a palpable swelling in the affected breast whereas mastalgia (38.5%), discharge from nipple, and ulcer were the other common complaints. The right breast was more commonly involved (66.7%), and upper and outer quadrant was the most common location of the tumor (74.4%) followed by upper and inner quadrant (8%). In 13% of the cases, more than one quadrant was involved. Most cases (69%) in this study received four to six cycles of neoadjuvant chemotherapy whereas 31% of cases received more than six cycles of chemotherapy. AC regimen (doxorubicin, cyclophosphamide followed by paclitaxel) was the most common (26 cases, 64.1%) chemotherapy regimen received. The second most common (8 cases, 20.5%) regimen received was CAF (cyclophosphamide, doxorubicin, 5-fluorouracil) followed by CEF (cyclophosphamide, epirubicin, 5-fluorouracil) regimen (6 cases, 15.38%). The mean size of the tumor before induction of chemotherapy was found to be 3.75 cm whereas after chemotherapy it was 1.75 cm (p < 0.001). The tumor size decreased consistently after neoadjuvant chemotherapy. Stage II breast carcinoma was found to be the most predominant (61.5%) stage with stage IIA accounting for up to 38.5% of the cases, and 23% of the cases were found to be in stage III. It was observed that younger women had an aggressive breast cancer with stage III as the predominant stage noted. IDC-NOC (infiltrating ductal carcinoma–not otherwise specified) (Figs. 123456789101112131415) was the most common histologic type both before (94.87%) and after (76.92%) therapy. One case of lobular carcinoma and one case of metaplastic carcinoma (Figs. 161718) were also found. Thirty-two of the 39 tumors changed grade to either a higher or lower grade (p < 0.05). On histopathologic examination, pathologic complete response (pCR) (Figs. 192021) was observed in 18% of the cases whereas 15% showed pathologic partial response (pPR) and 66.7% cases had a stable disease (SD) with no progression of disease (Table 1). The fundamental manifestation of the effect of therapy was an obvious decrease in tumor cellularity. Intracellular changes commonly noted after chemotherapy-included nuclear enlargement, hyperchromasia, and increased nuclear-to-cytoplasmic (N:C) ratio that were found in up to 85% of the cases, followed by presence of prominent nucleoli and karyorrhexis/karyolysis noted in 70 to 75% of the cases (Table 2). The predominant stromal changes seen post-therapy were necrosis (74.4%), fibrosis (64.1%) (Figs. 234561011192021), desmoplasia (59%), and degenerative changes (33.3%). Other significant stromal changes included mucinous changes, hyalinization of walls of blood vessels, (Fig. 2) neo-angiogenesis, calcific deposits (Figs. 31011), loss of cellular architecture, and appearance of chronic inflammatory cell infiltration (Figs. 2021) and hemosiderin-laden macrophages (Figs. 456), giant cells, and histiocytes (Table 3).

Fig. 1 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Tumor cells (H&E, 40x).
Fig. 1 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Tumor cells (H&E, 40x).
Fig. 2 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Lymph nodal tissue showing residual tumor cells with areas of fibrosis and hyalinization (H&E, 100x).
Fig. 2 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Lymph nodal tissue showing residual tumor cells with areas of fibrosis and hyalinization (H&E, 100x).
Fig. 3 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Residual tumor cells showing areas of fibrosis and calcification (H&E, 200x).
Fig. 3 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Residual tumor cells showing areas of fibrosis and calcification (H&E, 200x).
Fig. 4 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Residual tumor cells showing hemosiderin laden macrophages along with areas of fibrosis (H&E, 40x).
Fig. 4 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Residual tumor cells showing hemosiderin laden macrophages along with areas of fibrosis (H&E, 40x).
Fig. 5 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Residual tumor cells showing hemosiderin laden macrophages along with areas of fibrosis (H&E, 100x).
Fig. 5 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Residual tumor cells showing hemosiderin laden macrophages along with areas of fibrosis (H&E, 100x).
Fig. 6 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Residual tumor cells showing hemosiderin laden macrophages along with areas of fibrosis (H&E, 200x).
Fig. 6 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Residual tumor cells showing hemosiderin laden macrophages along with areas of fibrosis (H&E, 200x).
Fig. 7 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Perinodal fat infiltration of tumor cells (H&E, 100x).
Fig. 7 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Perinodal fat infiltration of tumor cells (H&E, 100x).
Fig. 8 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Residual tumor cells in lymph nodal tissue (H&E, 200x).
Fig. 8 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Residual tumor cells in lymph nodal tissue (H&E, 200x).
Fig. 9 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Residual tumor cells in lymph nodal tissue (H&E, 400x).
Fig. 9 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Residual tumor cells in lymph nodal tissue (H&E, 400x).
Fig. 10 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Residual tumor cells showing areas of fibrosis and calcification (H&E, 100x).
Fig. 10 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Residual tumor cells showing areas of fibrosis and calcification (H&E, 100x).
Fig. 11 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Residual tumor cells showing areas of fibrosis and calcification (H&E, 200x).
Fig. 11 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Residual tumor cells showing areas of fibrosis and calcification (H&E, 200x).
Fig. 12 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Tumor in skeletal muscle (H&E, 40x).
Fig. 12 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Tumor in skeletal muscle (H&E, 40x).
Fig. 13 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Tumor in skeletal muscle (H&E, 200x).
Fig. 13 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Tumor in skeletal muscle (H&E, 200x).
Fig. 14 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Lymphovascular invasion (H&E, 100x).
Fig. 14 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Lymphovascular invasion (H&E, 100x).
Fig. 15 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Neural invasion (H&E, 200x).
Fig. 15 IDC-NOC: Infiltrating ductal carcinoma-not otherwise specified: Post-therapy evaluation of tumor: Neural invasion (H&E, 200x).
Fig. 16 Metaplastic carcinoma: Post-therapy evaluation of tumor: Metaplastic carcinoma with squamous differentiation (H&E, 40x).
Fig. 16 Metaplastic carcinoma: Post-therapy evaluation of tumor: Metaplastic carcinoma with squamous differentiation (H&E, 40x).
Fig. 17 Metaplastic carcinoma: Post-therapy evaluation of tumor: Metaplastic carcinoma with squamous differentiation (H&E, 100x).
Fig. 17 Metaplastic carcinoma: Post-therapy evaluation of tumor: Metaplastic carcinoma with squamous differentiation (H&E, 100x).
Fig. 18 Metaplastic carcinoma: Post-therapy evaluation of tumor: Squamous pearls (H&E, 200x).
Fig. 18 Metaplastic carcinoma: Post-therapy evaluation of tumor: Squamous pearls (H&E, 200x).
Fig. 19 pCR: pathologic complete response of breast cancer: Post-therapy evaluation of tumor: Fibrotic stroma (H&E, 40x).
Fig. 19 pCR: pathologic complete response of breast cancer: Post-therapy evaluation of tumor: Fibrotic stroma (H&E, 40x).
Fig. 20 (A,B) pCR: pathologic complete response of breast cancer: Post-therapy evaluation of tumor: Granulation tissue with areas of fibrosis, chronic inflammatory cell infiltration and hemorrhage.
Fig. 20 (A,B) pCR: pathologic complete response of breast cancer: Post-therapy evaluation of tumor: Granulation tissue with areas of fibrosis, chronic inflammatory cell infiltration and hemorrhage.
Fig. 21 (A–C) Inflammatory response with areas of fibrosis and absence of tumor cells.
Fig. 21 (A–C) Inflammatory response with areas of fibrosis and absence of tumor cells.
Table 1 Carcinoma breast: comparison of effect of therapy

Effect of therapy

Obtained in study conducted by van der Wall et al18 (n = 62), n (%)

Obtained in study conducted by Burcombe et al13 (n = 99), n (%)

Obtained in study conducted by Moon et al25 (n = 82), n (%)

Obtained in study conducted by Sethi et al6 (n = 40), n (%)

Obtained in this study (n = 39), n (%)

pCR

13/62 (21)

8/99 (8)

21 (25)

4/40 (10)

7/39 (17.9)

pPR

42/62 (68)

30/99 (30)

49 (59)

12/40 (30)

6/39 (15.4)

pNR

7/62 (11)

61/99 (62)

12 (15)

24/40 (60)

26/39 (66.7)

Abbreviations: pCR, pathologic complete response; pNR, pathologic no response; pPR, pathologic partial response.

Table 2 Carcinoma breast: correlation of nuclear changes after neoadjuvant therapy

Nuclear response

Number of cases (%)

Nuclear enlargement

35 (89.7)

Hyperchromasia

34 (87.2)

Increased nuclear-to-cytoplasmic ratio

33 (84.6)

Presence of prominent nucleoli

30 (76.9)

Karyorrhexis/karyolysis

28 (71.8)

Pyknosis

23 (59)

Pleomorphic nuclei

24 (61.5)

Table 3 Carcinoma breast: correlation of stromal changes/response after neoadjuvant therapy

Stromal change/response

Number of cases (%)

Elastosis/collagenization

14 (35.9)

Fibrosis

25 (64.1)

Necrosis

29 (74.4)

Desmoplasia

23 (59)

Hyalinization of the walls of blood vessels

2 (5.1)

Calcification

9 (23.1)

Mucinous change

3 (7.7)

Foamy histiocytes

8 (20.5)

Cancerization of lobules

10 (25.6)

Angiogenesis

9 (23.1)

Giant cells

14 (35.9)

Atrophy of adjacent breast parenchyma

7 (17.9)

Degenerative changes

13 (33.3)

Hemosiderin-laden macrophages

7 (17.9)

Discussion

Breast cancer therapy causes morphologic alterations in the cancerous as well as the surrounding healthy tissue. However, more commonly changes are described after neoadjuvant chemotherapy (also, termed primary systemic therapy or pre-surgical therapy) in which chemotherapy and hormonal and targeted therapies are administered before the actual surgical excision of the tumor. To have a better insight about the histopathologic changes induced by chemotherapy on a breast cancer, this study analyzed a series of breast cancer cases before and after the therapy. Clinical and histopathologic details were collected from a total of 39 cases of breast carcinoma before and post-therapy, and the changes induced by the therapy were correlated. Overall, breast cancer is the second most common cancer with India contributing to approximately 7% of the global burden.4,5 Because age is one of the most important risk factor for breast cancers, the incidence of breast cancers is seen to increase with age with most women getting diagnosed with breast cancers after the age of 40 years in their lives. In this study, the median age of breast carcinoma patients was found to be 55 years in contrast to the studies conducted by Sethi et al6 and Faneyte et al7 wherein the median age of breast cancer patients was found to be 46 and 45 years, respectively. In this study, the most common chief complaint was a palpable swelling in the affected breast whereas mastalgia (38.5%), discharge from nipple, and ulcer were the other common complaints noted. The right breast was more commonly involved (66.7%), and upper and outer quadrant was the most common location of the tumor (74.4%) followed by upper and inner quadrant (8%). In 13% of the cases, more than one quadrant was involved. This was in concordance with most of the reported studies wherein upper and outer quadrant was the most common location of the tumor.6,8 Interestingly, in this study, the mean size of the tumor before induction of chemotherapy was found to be 3.75 cm whereas after chemotherapy it was 1.75 cm (p < 0.001), indicating a reduction in mean size of the tumor by 50% seen in both small and large tumors in contrast with the study conducted by Fisher et al9 wherein 70 to 80% of the patients demonstrated > 50% decrease in the mean size of the tumor. As it is a known fact that IDC-NOC is the predominant histologic type seen in any breast cancer, IDC-NOC was the most common histologic type seen both before (94.87%) and after (76.92%) therapy in this study whereas one case each of lobular carcinoma and metaplastic carcinoma was also reported in conjunction with most of the previous studies.6,10,11,12,13 Therapy and prognosis in breast cancers largely depend on the stage at which the cancer is diagnosed. Breast cancer stage is one of the most important prognostic indicators and significant determinant of the patient's overall survival. Stage II breast carcinoma was found to be the most predominant (61.5%) stage with stage IIA accounting for up to 38.5% of the cases, and 23% of the cases were found to be in stage III in this study. It was observed that younger women had an aggressive breast cancer with stage III as the predominant stage noted. These findings were in accordance with the numerous other studies conducted including the one conducted by Orucevic et al14 wherein stage II breast carcinoma was found to be the most predominant type. Similarly, stage II was the predominant stage reported in the studies conducted by Faneyte et al7 and von Minckwitz et al15 with 41% and 52% of the total cases presenting with stage II tumors, respectively. Narendra and Ray16 and Gajdos et al,17 however, reported most cases belonging to stage III in their studies in contrast to the aforementioned studies. Chemotherapy-induced morphologic changes were first described by Waller in 1960 when he described cytoplasmic swelling and vacuolation caused by administration of busulfan. Kennedy et al in 1990 further described such changes in patients with breast carcinoma when combination of tamoxifen and cytotoxic drug therapy was used. Since then, many studies have been published regarding the interaction of chemotherapeutic agents with tumor biology; however, there are very few studies that are detailed enough to include all the parameters such as necrobiotic changes, viability of the tumor cells and host tissue response to chemotherapeutics agents, etc., which could predict response to chemotherapy. In this study, an elaborative assessment of the cellular and stromal changes was done before and after the therapy. In the stromal changes, generalized increase in collagenization was significantly correlated with pathologic response and grades of tumor regression. The predominant stromal changes seen post-therapy included necrosis (74.4%), fibrosis (64.1%), desmoplasia (59%), and degenerative changes (33.3%) whereas mucinous changes, hyalinization of walls of blood vessels, neo-angiogenesis, calcific deposits, loss of cellular architecture, and appearance of chronic inflammatory cell infiltration and hemosiderinladen macrophages, giant cells, and histiocytes were the other significant stromal changes reported. Sethi et al6 reported generalized increase in mucinous and metaplastic changes left after chemotherapy. Honkoop et al12 also noticed similar mucinous changes left after chemotherapy. This was also in contrast to numerous other studies that did not report any significant histopathologic changes post-chemotherapy.12,18,19 Gazet et al20 also reported that lobular carcinoma was less responsive to chemotherapy due to high stromal content; however, the same could not be substantiated in this study because only one case each of lobular carcinoma and metaplastic carcinoma was reported in this study. The fundamental manifestation of the effect of therapy was an obvious decrease in tumor cellularity whereas the other prominent changes included nuclear enlargement, hyperchromasia, and increased N:C ratio that were found in up to 85% of the cases followed by presence of prominent nucleoli and karyorrhexis/karyolysis noted in 70 to 75% of the cases. Similar changes have also been observed independently in numerous other studies.6,14 Such cytologic changes are seen because of the cellular damage seen secondary to neoadjuvant chemotherapy as has been hypothesized in other studies and are variable in different tumors after the therapy, and the actual change might further differ on the chemotherapy regimen used.9,21,22 The true biologic significance of such cytopathic changes in residual breast cancers, however, has received little attention and is poorly understood.23,24 Various inflammatory infiltrates might also be seen due to host tissue response to the necrobiotic tumor such as appearance of chronic inflammatory cell infiltration and hemosiderin-laden macrophages, intense lymphocytic reactions, presence of plasma cells, and giant cells, and histiocytes as described in various studies.12,20,21,24,25 Stromal elastosis too is seen as a prominent change after therapy.7,11,12,20,21 In this study, response to chemotherapy was classified according to the International Union against Cancer (UICC) as pCR defined as absence of residual viable tumor cells (DCIS included), pPR as greater than 50% reduction in the number of viable tumor cells, SD as less than 50% reduction in the number of viable tumor cells, or no change and progressive disease (PD) as an increase of at least 25% of viable tumor cells. On histopathologic examination, pCR was observed in 18% of the cases in this study whereas 15% showed pPR and 66.7% cases had an SD with no progression of disease after therapy. pCR was seen in seven of the cases of IDC-NOC type of the tumor whereas one case each of lobular, metaplastic, and ductal carcinoma had pPR. Twenty-six cases revealed pNR whereas response in IDC-NOC type of tumor was not significantly different from that observed in invasive lobular carcinoma.

Conclusion

Breast cancer therapy causes morphologic alterations in the cancerous as well as the surrounding healthy tissue. The histopathologic interpretation in such cases, thus, requires a thorough knowledge of the cytologic and stromal changes rendered by the therapy. This ensures the role of the pathologists extremely important in correct diagnosis as well as grading of the tumor with a correct histopathologic interpretation for an effective and planned regimen of the therapy increasing prognosis and chances of survival of the affected patients rendering better clinical outcome and an effective patient care.

Acknowledgments

The authors would like to thank all the patients who contributed in the study without whom this study would not have been feasible.

Conflict of Interest

None declared.

FundingNone.

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